Istituto di Scienza e Tecnologie dell'Informazione     
Danti S., Ciofani G., Pertici G., Moscato S., D'Alessandro D., Ciabatti E., Chiellini F., Chiellini F., D'Acunto M., Mattoli V., Berrettini S. Boron nitride nanotube-functionalised myoblast/microfibre constructs: a nanotech-assisted tissue engineered platform for muscle stimulation. In: Journal of Tissue Engineering and Regenerative Medicine, vol. 9 (7) pp. 847 - 851. Wiley, 2015. [Online First 04 March 2014]
In this communication, we introduce boron nitride nanotube (BNNT)-functionalised muscle cell/microfibre mesh constructs, obtained via tissue engineering, as a three-dimensional (3D) platform to study a wireless stimulation system for electrically responsive cells and tissues. Our stimulation strategy exploits the piezoelectric behaviour of some classes of ceramic nanoparticles, such as BNNTs, able to polarize under mechanical stress, e.g. using low-frequency ultrasound (US). In the microfibre scaffolds, C2C12 myoblasts were able to differentiate into viable myotubes and to internalize BNNTs, also upon US irradiation, so as to obtain a nanotech-assisted 3D in vitro model. We then tested our stimulatory system on 2D and 3D cellular models by investigating the expression of connexin 43(Cx43), as a molecule involved in cell crosstalk and mechanotransduction, and myosin, as a myogenic differentiation marker. Cx43 gene expression revealed a marked model dependency. In control samples (without US and/or BNNTs), Cx43 was upregulated under 2D culture conditions (10.78▒1.05-fold difference). Interactions with BNNTs increased Cx43 expression in 3D samples. Cx43 mRNA dropped in 2D under the 'BNNTs+US' regimen, while it was best enhanced in 3D samples (3.58▒1.05 vs 13.74▒1.42-fold difference, p=0.0001). At the protein level, the maximal expressions of Cx43 and myosin were detected in the 3D model. In contrast with the 3D model, in 2D cultures, BNNTs and US exerted a synergistic depletive effect upon myosin synthesis. These findings indicate that model dimensionality and stimulatory regimens can strongly affect the responses of signalling and differentiation molecules, proving the importance of developing proper in vitro platforms for biological modelling.
In questo lavoro, noi introduciamo una piattaforma 3D composta da cellule di muscolo funzionalizzate con nanotubi di nitruro di boro (BNNT) e microfibre per studiare un sistema di stimolazione wireless per cellule che rispondono a stimoli elettrici. I risultati ottenuti mostrano che il sistema 3D condiziona fortemente la capacitÓ di differenziamento delle cellule stesse.
URL: http://onlinelibrary.wiley.com/doi/10.1002/term.1878/abstract
DOI: 10.1002/term.1878
Subject Boron nitride nanotubes (BNNTs)
Three-dimensional (3D) model
I.4.5 Reconstruction
I.4.10 Image Representation
I.6.1 Simulation Theory
62H35 Image analysis

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