Istituto di Scienze Marine     
Vitturi R., Colomba M., Vizzini S., Libertini A., Barbieri R., Mazzola A. Chromosomal location polymorphism of major rDNA sites in two Mediterranean populations of the killifish Aphanius fasciatus (Pisces : Cyprinodontidae). In: Micron, vol. 36 (3) pp. 243 - 246. Pergamon-elsevier Science Ltd, 2005.
The chromosomes of the Mediterranean killifish, Aphanius fasciatus from two populations, the Lagoon of Venice (LV, 15 specimens) and the Lagoon 'Stagnone di Marsala' (Sicily) (SM, 48 specimens), have been investigated using conventional Ag-staining and fluorescent in situ hybridization (FISH) with 18S rDNA probe. The two methods revealed variation in the number of major rDNA sites ranging from 8 to 14 (LV) and from I to 4 (SM) per individual. The fact that each individual possessed its own number of sites implies that observed variation was structural. Moreover, overlapping of silver staining and FISH patterns demonstrated that all ribosomal genes were transcriptionally active in each specimen. (c) 2005 Elsevier Ltd. All rights reserved.
DOI: 10.1016/j.micron.2004.11.006
Subject karyotype polymorphism
in situ hybridization
Aphanius fasciatus
Mediterranean sea

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