Istituto di Scienze Marine     
Piazza V., Ferioli A., Giacco E., Melchiorre N., Valenti A., Del Prete F., Biandolino F., Dentone L., Frisenda P., Faimali M. A standardization of Amphibalanus (Balanus) amphitrite (Crustacea, Cirripedia) larval bioassay for ecotoxicological studies. In: Ecotoxicology and Environmental Safety, vol. 79 pp. 134 - 138. Elsevier, 2012.
A series of 10 intra-laboratory bioassays and an inter-laboratory assay involving nine laboratories,were performed to validate the use of Amphibalanus amphitrite larvae as test organisms for ecotoxicological studies.The standardization protocol utilized Cadmium Nitrate as a reference toxicant and larval immobilization(after24and48h)as the end-point.The statistical data analysis showed high homogeneity in intra-laboratory EC50 values among bioassay repetitions(coefficient of variation (CV)15.8% after 24h and 16.9% after 48h);also CV values obtained in inter laboratory comparison exercise were not higher than the 50%,variation coefficient mentioned in the ISO standards for the precision of inter-laboratory assays.The results demonstrated that the protocol implemented for the balanus larval bioassay comply with the international standards both for intra-and inter-laboratory precision.
DOI: 10.1016/j.ecoenv.2011.12.014
Subject ecotoxicology

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