PUMA
Istituto di Fisiologia Clinica     
Andreassi M. G., Cioppa A., Manfredi S., Neri M. G., Foffa I., Picano E. N-acetyl cysteine reduces chromosomal DNA damage in circulating lymphocytes during cardiac catheterization procedures: A pilot study. In: International Journal of Cardiology, vol. 21 pp. 1 - 4. Elsevier, 2011.
 
 
Abstract
(English)
Background: N-acetylcysteine (NAC) is considered a promising radio-protector for its antioxidant and anticarcinogenic properties. We examined the ability of NAC to confer protection against radiation-induced chromosomal DNA damage during cardiac catheterization procedures. Methods: Sixty-five patients (52 males, age 64.411.9 years) undergoing invasive cardiovascular procedures (peripheral transluminal angioplasty, n=45; cardiac resynchronization therapy, n=15 and ablation therapy n=5) were enrolled: 35 patients (26 males, age 63.411.1 years) received the standard hydration protocol consisting of intravenous isotonic saline for 12 h after catheterization (Group I), and 30 patients (26 males, age 65.512.9 years) received a clinically driven double intravenous dose of NAC (6 mg/kg/h diluted in 250 mL of NaCl 0.9%) for 1 h before and a standard dose (6 mg/kg/h diluted in 500 mL of NaCl 0.9%) for 12 h following catheterization (Group II). Micronucleus assay (MN) was performed as biomarker of chromosomal DNA damage before, 2 and 24 h after the radiation exposure. Dose-area product (DAP; Gy cm2) was assessed as physical measure of radiation load. Results: DAP was higher in NAC-treated patients (I=54.723.6 vs II=126.279.2 Gy cm2, p=0.0001). MN frequency was 13.74.7 at baseline and showed a significant rise at 2 h (18.06.8 p=0.01) and 24 h (17.65.9, p=0.03) in the Group I. There was no significant increase ofMNin the Group II (13.77.0, 15.5 6.0 and 14.96.3 for baseline, 2 h and 24 h respectively, p=0.4). Conclusion: NAC treatment given to prevent contrast-induced nephropathy may also reduce DNA damage induced by ionizing radiation exposure during cardiac catheterization procedures.
URL: http://www.elsevier.com
DOI: 10.1016/j.ijcard.2011.05.001
Subject Ionizing radiation
NAC
DNA damage


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