PUMA
Istituto di Fisiologia Clinica     
Del Carratore R., Gervasi P. G., Contini M. P., Beffy P., Maserti E., Giovannetti G., Brondolo A., Longo V. Expression and characterization of two new alkane-inducible cytochrome P450s from Trichoderma harzianum. In: Biotechnology Letters, vol. 33 pp. 1201 - 1206. Springer, 2011.
 
 
Abstract
(English)
Abstract The inducibility CYPs by various carbon sources, including some n-alkanes and fatty acids, has been studied in Trichoderma harzianum. It was observed that n-dodecane and a mixture of fatty acids were good inducers of total CYP content and ω-hydroxylase of lauric acid, a marker for ω-hydroxylation of n-alkanes. By RACE it was isolated a cDNA containing an open reading frame of 1520 bp which encoded a CYP52 protein of 520 amino acids. Further, another n-alkane inducible CYP was identified in a library of T. harzianum by LC-MS/Ms analysis of a microsomal protein band induced by n-dodecane exposure. Thus, the filamentous fungus T. harzianum is expected to have a CYP dependent conversion of alkanes to fatty acids and their incorporation into cellular lipids.
Subject Cytochrome P450 induction


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