Istituto di Fisiologia Clinica     
Del Carratore R., Gervasi P. G., Contini M. P., Beffy P., Maserti E., Giovannetti G., Brondolo A., Longo V. Expression and characterization of two new alkane-inducible cytochrome P450s from Trichoderma harzianum. In: Biotechnology Letters, vol. 33 pp. 1201 - 1206. Springer, 2011.
Abstract The inducibility CYPs by various carbon sources, including some n-alkanes and fatty acids, has been studied in Trichoderma harzianum. It was observed that n-dodecane and a mixture of fatty acids were good inducers of total CYP content and ω-hydroxylase of lauric acid, a marker for ω-hydroxylation of n-alkanes. By RACE it was isolated a cDNA containing an open reading frame of 1520 bp which encoded a CYP52 protein of 520 amino acids. Further, another n-alkane inducible CYP was identified in a library of T. harzianum by LC-MS/Ms analysis of a microsomal protein band induced by n-dodecane exposure. Thus, the filamentous fungus T. harzianum is expected to have a CYP dependent conversion of alkanes to fatty acids and their incorporation into cellular lipids.
Subject Cytochrome P450 induction

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