PUMA
Istituto di Fisiologia Clinica     
Del Ry S., Cabiati M., Lionetti V., Colotti C., Maltinti M., Emdin M., Recchia F. A., Giannessi D. Sequencing and cardiac expression of natriuretic peptide receptor 2 (NPR-B) in Sus Scrofa. In: Peptides, vol. 28 pp. 1390 - 1396. doi:10.1016/j.peptides.2007.05.003. Elsevier, 2007.
 
 
Abstract
(English)
The cardiovascular actions of the C-type natriuretic peptide (CNP) are mainly mediated by the interaction with natriuretic peptide receptor-B (NPR-B). The aim of this study was to identify the sequence of NPR-B in Sus Scrofa, which is not present in GenBank, to verify the expression of NPR-B in the different cardiac chambers of normal pigs and evaluate its homology with murine and human species. Using the guanidinium thyocyanate-phenolchloroform method, we extracted total RNA from samples obtained from heart of mouse and from the atrium, ventricle, and septum of normal pigs. Pig NPR-B mRNA was sequenced using polymerase chain reaction primers designed from mouse consensus sequences. Sus Scrofa natriuretic peptide receptor 2 mRNA, 1-396 bp, was submitted to GenBank (accession number DQ487044). The presence of NPR-B at mRNA level was detected in all the cardiac chambers; moreover, the bands obtained from pig cardiac tissue shared a 93% sequence homology with a region of the mouse NPR-B and a 95% sequence homology with Homo sapiens. Therefore, NPR-B sequencing provides a new tool to investigate the role of CNP under physiological and pathological conditions in the experimental and clinical setting.
Subject Natriuretic peptide receptor-B
Sus Scrofa
mRNA
Cardiac tissue


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