Istituto di Fisiologia Clinica     
Del Ry S., Cabiati M., Lionetti V., Colotti C., Maltinti M., Emdin M., Recchia F. A., Giannessi D. Sequencing and cardiac expression of natriuretic peptide receptor 2 (NPR-B) in Sus Scrofa. In: Peptides, vol. 28 pp. 1390 - 1396. doi:10.1016/j.peptides.2007.05.003. Elsevier, 2007.
The cardiovascular actions of the C-type natriuretic peptide (CNP) are mainly mediated by the interaction with natriuretic peptide receptor-B (NPR-B). The aim of this study was to identify the sequence of NPR-B in Sus Scrofa, which is not present in GenBank, to verify the expression of NPR-B in the different cardiac chambers of normal pigs and evaluate its homology with murine and human species. Using the guanidinium thyocyanate-phenolchloroform method, we extracted total RNA from samples obtained from heart of mouse and from the atrium, ventricle, and septum of normal pigs. Pig NPR-B mRNA was sequenced using polymerase chain reaction primers designed from mouse consensus sequences. Sus Scrofa natriuretic peptide receptor 2 mRNA, 1-396 bp, was submitted to GenBank (accession number DQ487044). The presence of NPR-B at mRNA level was detected in all the cardiac chambers; moreover, the bands obtained from pig cardiac tissue shared a 93% sequence homology with a region of the mouse NPR-B and a 95% sequence homology with Homo sapiens. Therefore, NPR-B sequencing provides a new tool to investigate the role of CNP under physiological and pathological conditions in the experimental and clinical setting.
Subject Natriuretic peptide receptor-B
Sus Scrofa
Cardiac tissue

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