Istituto di Fisiologia Clinica     
Vassalle C., Landi P., Boni C., Zucchelli G. C. Oxidative stress evaluated using an automated method for hydroperoxide estimation in patients with coronary artery disease. In: Clinical Chemistry and Laboratory Medicine, vol. 45 (3) pp. 367 - 371. DOI 10.1515/CCLM.2007.073. Walter de Gruyter Berlin New York, 2007.
Background: Reactive oxygen species (ROS) have a major role in the pathogenesis of different chronic and degenerative diseases, including atherosclerosis. However, the lack of feasible and reliable methods limits the spread of oxidative stress estimation for routine application in clinical chemistry laboratories. We have recently evaluated the analytical characteristics of an automated test for the measurement of hydroperoxides (HP) and its performance in determining oxidative stress levels in a general population. In this study we aimed to apply this method for the evaluation of oxidative stress in a cohort of patients with coronary artery disease (CAD). Methods: 69 patients with angiographically verified CAD and 34- age and sex matched- control subjects were enrolled in the study. Results: HP were found higher in patients with CAD (p<0.01), significantly increasing with severity of the disease (p<0.01). HP resulted also higher in subjects with diabetes dyslipidemia or C-reactive protein >1.5 mg/dl. A significant positive correlation was observed between glucose and HP levels. In the multivariate model, diabetes (odds ratio, OR=3.5, 95%CI 1.2-10, p<0.05) and CAD (OR=5.7, CI 1.1-28.5, p<0.05) resulted independent determinants for the 75th HP percentile. Conclusion: Results obtained with this method largely reproduce those found by using other oxidative stress biomarkers, but it is faster, easy to perform and does not require skilled operators or complex instrumentation, thus representing a reliable procedure which might constitute a feasible tool for oxidative stress estimation in the cardiovascular setting.
Subject atherosclerosis

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