Istituto di Fisiologia Clinica     
Ahluwalia A., Vozzi G., Bianchi F., Vozzi F. Bioreactors for the study of endothelial cells in force fields. In: Biomedicine & Pharmacotherapy, vol. 60 pp. 468 - 468. elsevier, 2006.
Cardiovascular disease (CVD), such as hypertension, is one of the principal causes of death in the western world. A great amount of effort is being addressed towards the understanding of the pathological processes involved in the genesis of hyper- tension. Endothelial cells covered the internal side of blood vessels and play an essential role in the regulation of vessel homeos- tasis, of flow and of blood pressure in the circulator system. The endothelium is a dynamic system which is able to answer to different physical and chemical conditions through the production of many vaso-active substances such as: endothelins and nitric oxide. These molecules act on the smooth muscle cells which form the vessel side, in a paracrine manner. For example the endothelin system has been demonstrated to participate in the pathophysiology of numerous conditions, including hypertension, atherosclerosis, coronary artery dis- ease, heart failure, subarachnoid haemorrhage, diabetes, pri- mary pulmonary hypertension, pulmonary fibrosis, sclero- derma, renal failure, prostate cancer and its metastasis, etc. This study concerns the effect of the variation of pressure and shear stress on an endothelial cell culture by measuring endothelin 1 (ET-1) and NOx levels, to understand the beha- viour of the endothelium behaviour under different types of force. The pressure and the shear stress conditions were generated using a new type of bioreactor: a Laminar Flow and Pressure Controlled Bioreactor, developed at the Interdipartimental Cen- tre of Research "E. Piaggio" of Pisa University. The results show that elevated hydrostatic pressures lead to a reduction in ET-1 whereas NOx levels are unaffected. On the other hand, when a shear stress is applied, both ET-1 and NOx levels are altered. The results are discussed in terms of the role the two forces play on the intima.
Subject bioreactor

Icona documento 1) Download Document PDF

Icona documento Open access Icona documento Restricted Icona documento Private


Per ulteriori informazioni, contattare: Librarian http://puma.isti.cnr.it

Valid HTML 4.0 Transitional