PUMA
Istituto di Fisiologia Clinica     
Ahluwalia A., Vozzi G., Bianchi F., Vozzi F. Bioreactors for the study of endothelial cells in force fields. In: Biomedicine & Pharmacotherapy, vol. 60 pp. 468 - 468. elsevier, 2006.
 
 
Abstract
(English)
Cardiovascular disease (CVD), such as hypertension, is one of the principal causes of death in the western world. A great amount of effort is being addressed towards the understanding of the pathological processes involved in the genesis of hyper- tension. Endothelial cells covered the internal side of blood vessels and play an essential role in the regulation of vessel homeos- tasis, of flow and of blood pressure in the circulator system. The endothelium is a dynamic system which is able to answer to different physical and chemical conditions through the production of many vaso-active substances such as: endothelins and nitric oxide. These molecules act on the smooth muscle cells which form the vessel side, in a paracrine manner. For example the endothelin system has been demonstrated to participate in the pathophysiology of numerous conditions, including hypertension, atherosclerosis, coronary artery dis- ease, heart failure, subarachnoid haemorrhage, diabetes, pri- mary pulmonary hypertension, pulmonary fibrosis, sclero- derma, renal failure, prostate cancer and its metastasis, etc. This study concerns the effect of the variation of pressure and shear stress on an endothelial cell culture by measuring endothelin 1 (ET-1) and NOx levels, to understand the beha- viour of the endothelium behaviour under different types of force. The pressure and the shear stress conditions were generated using a new type of bioreactor: a Laminar Flow and Pressure Controlled Bioreactor, developed at the Interdipartimental Cen- tre of Research "E. Piaggio" of Pisa University. The results show that elevated hydrostatic pressures lead to a reduction in ET-1 whereas NOx levels are unaffected. On the other hand, when a shear stress is applied, both ET-1 and NOx levels are altered. The results are discussed in terms of the role the two forces play on the intima.
Subject bioreactor


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