Istituto di Fisiologia Clinica     
Campolo J., De Chiara B., Caruso R., De Maria R., Sedda V., Della Noce C., Parolini M., Cighetti G., Penco S., Baudo F., Parodi O. Methionine challenge paradoxically induces a greater activation of the antioxidant defence in subjects with hyper- vs normohomocysteinemia. In: Free Radical Research, vol. 40 (9) pp. 929 - 935. Taylor & Francis, 2006.
To determine whether chronic hyperhomocysteinemia induced post-methionine loading (PML) is associated with different response in the aminothiol redox state and oxidative stress versus normohomocysteinemia post-methionine loading (PML) , we assessed PML plasma thiols, vitamins, free malondialdehyde (MDA), and blood reduced glutathione (GSH) in 120 consecutive subjects (50 [35-56] years, 83 males), divided into two groups according to PML plasma total Hcy : < 35 ÁM (Group 1, n = 65) or and ? 35 ÁM (Group 2, n = 55). In the group as a whole, Plasma plasma reduced cysteine and cysteinylglycine, blood reduced GSH (all p for time = 0.0001) and plasma total GSH (p for time = 0.001) increased from baseline to PML. MDA values were unchanged. Group 1 and 2 differed in blood reduced GSH (p for group = 0.004, higher in Group 2), and MDA levels (p for group = 0.024, lower in Group 2). The oxidative stress induced by methionine challenge seems to be opposed by scavenger molecules activation, namely GSH, and lipid peroxidation does not increase. This mechanism paradoxically appears to be more efficient in hyperhomocysteinemic subjects.
Subject Homocysteine
Oxidative stress
Methionine loading

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