Istituto di Fisiologia Clinica     
Andreu Y., Baldini F., Domenici C., Giannetti A., Masci D., Mencaglia A. Optical fibre sensor for photosynthetic herbicides detection by time-resolved absorption. In: Proceedings of the Society of Photo-Optical Instrumentation Engineers, vol. 5270 pp. 31 - 37. SPIE Society, 2004.
The present work describes a new sensing system for the detection of photosynthetic herbicides in water based on the use of the reaction centre (RC) isolated from Rhodobacter sphaeroides, a purple bacteria. RC is a trans-membrane protein complex with one characteristic: stationary and excited states have different absorption properties. The presence of herbicides affects the path followed by the protein to return to the stationary state. Therefore the herbicides could be detected by monitoring the temporal changes of absorption following optical excitation. An optoelectronic system has been realised for this purpose. A diodeIt at 860 nm and a hybrid photodetector are used as excitation sourcce and detector, respectively. Optical fibres couple the optoelectronic system to a 5 cm-long cell containing the RC solution. The system has been used for the detection of five different photosynthetic herbicides: diuron, atrazine, terbutryn, terbuthylazine and simazine. The detection limit of every herbicide has been obtained: 0.5 microM for terbutryn 1 microM for atrazine and terbuthylazine and 10 microM for diuron and simazine.
Subject Optical fibre

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