PUMA
Istituto di Fisiologia Clinica     
Carluccio M. A., Siculella L., Ancora M. A., Massaro M., Scoditti E., Storelli C., Visioli F., Distante A., De Caterina R. Olive oil and red wine antioxidant polyphenols inhibit endothelial activation antiatherogenic properties of Mediterranean diet phytochemicals. In: Arteriosclerosis Thrombosis and Vascular Biology, vol. 23 pp. 622 - 629. american heart association, 2003.
 
 
Abstract
(English)
Objective- Epidemiology suggests that Mediterranean diets are associated with reduced risk of cardiovascular disease. Because monocyte adhesion to the endothelium is crucial in early atherogenesis, we evaluated whether typical olive oil and red wine polyphenols affect endothelial-leukocyte adhesion molecule expression and monocyte adhesion. Methods and Results- Phytochemicals in olive oil and red wine, including oleuropein, hydroxytyrosol, tyrosol, elenolic acid, and resveratrol, with or without antioxidant activity, were incubated with human umbilical vein endothelial cells for 30 minutes, followed by co-incubation with bacterial lipopolysaccharide or cytokines to trigger adhesion molecule expression. At nutritionally relevant concentrations, only oleuropein, hydroxytyrosol, and resveratrol, possessing a marked antioxidant activity, reduced monocytoid cell adhesion to stimulated endothelium, as well as vascular cell adhesion molecule-1 (VCAM-1) mRNA and protein by Northern analysis and cell surface enzyme immunoassay. Reporter gene assays with deletional VCAM-1 promoter constructs indicated the relevance of nuclear factor-{kappa}B, activator protein-1, and possibly GATA binding sites in mediating VCAM-1 transcriptional inhibition. The involvement of nuclear factor-{kappa}B and activator protein-1 was finally demonstrated at electrophoretic mobility shift assays. Conclusions- Olive oil and red wine antioxidant polyphenols at nutritionally relevant concentrations transcriptionally inhibit endothelial adhesion molecule expression, thus partially explaining atheroprotection from Mediterranean diets.
URL: http://atvb.ahajournals.org/cgi/reprint/23/4/622
Subject atherosclerosis
adhesion molecules
VCAM-1
polyphenols
Mediterranean diets


Icona documento 1) Download Document PDF


Icona documento Open access Icona documento Restricted Icona documento Private

 


Per ulteriori informazioni, contattare: Librarian http://puma.isti.cnr.it

Valid HTML 4.0 Transitional