PUMA
Istituto di Fisiologia Clinica     
Sabatino L., Chopra I. J., Tanavoli S., Iacconi P., Iervasi G. A radioimmunoassay for type I iodothyronine 5'-monodeiodinase in human tissues. In: Thyroid, vol. 11 (08) pp. 733 - 739. Mary Ann Liebert, Inc. publishers, 2001.
 
 
Abstract
(English)
We have developed a sensitive, specific and reproducible radioimmunoassay (RIA) for measurement of human type I monodeiodinase (5'-DI) protein. Anti-5'-DI antibody was produced by immunization of rabbits with a conjugate of bovine serum albumin and a 16 amino acid synthetic peptide, corresponding to a portion of the carboxy-terminal region of the human 5'-DI (PI-99). In a final dilution of 1:500, our anti-5'-DI antibody bound about 30%-35% of a tracer amount of 125I-PI-99. The detection threshold of the RIA approximated 0.4 pmol PI-99 or an equivalent amount of 0.4 pmol 5'-DI. The coefficient of variation averaged 5% within an assay and 14% between assays. Dose-response curves of tissue proteins were essentially parallel to that of PI-99. In a total number of 35 normal human tissue samples, the mean ( standard deviation [SD], picomole per milligram of protein [pmol]) 5'-DI content was 25 6.7 in kidney, it was significantly lower (p < 0.05) in liver at 3.9 1.1, 2.8 0.8 in intestine, 2.3 0.98 in adrenal, 4.2 2.5 in skeletal muscle, 3.8 1.4 in heart and 2.6 2.4 in thyroid; it was 1.4 0.3 in Graves' thyroid. Our data suggest that (1) 5'-DI is distributed widely among human tissues; (2) kidney is the tissue most enriched with 5'-DI; (3) 5'-DI content in the thyroid is not increased in Graves' disease.
URL: http://www.liebertonline.com/doi/pdf/10.1089/10507250152484565
Subject Radioimmunoassay
Iodothyronine
Human


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