Istituto di Fisiologia Clinica     
Glaser M., Brown D. J., Law M. P., Iozzo P., Waters S. L., Poole K., Knickmeier M., Camici P. G., Pike V. W. Preparation of no-carrier-added [124I]A14-iodoinsulin as a radiotracer for positron emission tomography. In: Journal of Labelled Compounds & Radiopharmaceuticals, vol. 44 pp. 465 - 480. Wiley & Sons, 2001.
A14-Iodoinsulin is a close biochemical and pharmacological mimic of insulin. Human insulin was labelled in its A chain tyrosine-14 residue by direct iodination with the positron-emitter iodine-124 (+=25.6%; t1/2=4.15 days) to provide a radiotracer for imaging with positron emission tomography (PET). Several reagents were compared for conversion of cyclotron-produced [124I]iodide into a reactive species for the labelling reaction. Radiochemical yields from the use of Iodo-Gen®, Chloramine-T, N-bromosuccinimide or lactoperoxidase-hydrogen peroxide were similar [35% (n=1), 33±9% (n=10), 32±11% (n=25) or 33% (n=2), respectively]. [124I]A14-Iodoinsulin was separated from unreacted insulin and radioactive by-products by tandem reverse phase HPLC and rapidly formulated for intravenous injection by adsorption on a Sep-Pak tC18-Plus® cartridge, followed by elution with 10 mM hydrochloric acid-ethanol (1:1 v/v, 1 ml). This radiotracer can now be obtained in useful radioactivities at high effective specific radioactivity and is now being applied to PET studies of its biodistribution in living subjects.
URL: http://www3.interscience.wiley.com/cgi-bin/fulltext/81502621/PDFSTART
Subject radiotracer
human insulin

Icona documento 1) Download Document PDF

Icona documento Open access Icona documento Restricted Icona documento Private


Per ulteriori informazioni, contattare: Librarian http://puma.isti.cnr.it

Valid HTML 4.0 Transitional