Istituto di Fisiologia Clinica     
De Caterina R., Basta G., Madonna R., Statile D., Zimarino M., Gallina S., Procopio A. D. Endothelial cell transfections:a way for studying promoter activity of genes involved in atherogenesis. In: Italian Heart Journal, vol. 01 (supplement 3) pp. s55 - s58. CEPI - AIM Group, 2000.
By gene therapy we call the entire array of strategies by which a nucleic acid, usually DNA, is administered with the aim of modifying the genetic repertoire of target cells1. The aim of such an approach is to change the phenotypic response of the target cell, either through the synthesis of the gene product (the protein) encoded by the newly introduced nucleic acid, or through a change in the expression of a target gene. The former strategy utilizes the transcription and translation of the gene introduced, and thus exploits the cellular transcription and translation machinery allowing the production, from one single gene, of several copies of messenger RNA (mRNA), and from each of these several copies of the encoded protein. With the latter strategy, we aim at an interference with the mechanisms of transcription and translation. While the latter strategy may use, according to the case, either DNA or RNA, the nucleic acid is always DNA in the former.
URL: http://www.italheartj.org/pdf_files/20000214.pdf
Subject Endothelial

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