Istituto di Fisiologia Clinica     
Kusmic C., Picano E., Busceti C. L., Petersen C., Barsacchi R. The antioxidant drug dipyridamole spares the vitamin E and thiols in red blood cells after oxidative stress. In: Cardiovascular Research, vol. 47 (03) pp. 510 - 514. elsevier, 2000.
Objective: To test the antioxidant effect of therapeutic doses of dipyridamole on cellular membranes, human erythrocytes were chosen 21 as an appropriate model to study oxidative stress induced by cumene hydroperoxide because of their high content in heme-Fe . 21 Methods: The oxidative stress was induced by incubation with 160 mmol l cumene hydroperoxide and expressed by three main factors: lipid peroxidation by means of kinetics of decrease in fluorescence emission of the probe incorporated in the cell membranes, vitamin E 21 oxidation and intracellular thiol content. The concentrations of dipyridamole tested (2-20 mmol l ) did not exceed pharmacological 21 doses. Results. After 7 min of incubation at 258C with the oxidant and 20 mmol l dipyridamole thiol content was 50.1%62.6 compared with 31.5%62.4 in the absence of the drug. After 12 min vitamin E content was 88.3%62.3 compared with 64.7%63.4 of untreated cells in the absence of dipyridamole. Dipyridamole added 5 min after the oxidation reaction suppressed the fluorescence decrease for a time proportional to the drug concentration. Conclusions. Thus, at clinically realistic doses dipyridamole shows a concentration-dependent antioxidant effect. It protects membranes from oxidation and spares the antioxidant power of erythrocytes.
URL: http://scienceserver.cilea.it/cgi-bin/sciserv.pl?collection=journals&journal=00086363&issue=v47i0003&article=510_taddstrbcaos&form=pdf&file=file.pdf
Subject Free radicals

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