Istituto di Biofisica     
Podda A., Checcucci G., Mouhaya W., Centeno D., Rofidal V., Del Carratore R., Luro F., Morillon R., Ollitrault P., Maserti B. E. Salt-stress induced changes in the leaf proteome of diploid and tetraploid mandarins with contrasting Na+ and Cl− accumulation behaviour. In: Journal of Plant Physiology, vol. 170 pp. 1101 - 1112. Elsevier, 2013.
out in parallel on two citrus genotypes ('Cleopatra' and 'Willow leaf' mandarins), which differ for Na+ and Cl− accumulation, and their cognate autotetraploids (4). Using two-dimensional electrophoresis approximately 910 protein spots were reproducibly detected in control and salt-stressed leaves of all genotypes. Among them, 44 protein spots showing significant variations at least in one genotype were subjected to mass spectrometry analysis for identification. Salt-responsive proteins were involved in several functions, including photosynthetic processes, ROS scavenging, stress defence, and signalling. Genotype factors affect the salt-responsive pattern, especially that of carbon metabolism. The no ion accumulator 'Cleopatra' mandarin genotype showed the highest number of salt-responsive proteins, and up-regulation of Calvin cycle-related proteins. Conversely the ion accumulator 'Willow leaf' mandarin showed high levels of several photorespiration-related enzymes. A common set of proteins (twelve spots) displayed higher levels in salt-stressed leaves of 2 and 4 'Cleopatra' and 4 'Willow leaf' mandarin. Interestingly, antioxidant enzymes and heat shock proteins showed higher constitutive levels in 4 'Cleopatra' mandarin and 4 'Willow leaf' mandarin compared with the cognate 2 genotype. This work provides for the first time information on the effect of 8 weeks of salt stress on citrus genotypes contrasting for ion accumulation and their cognate autotetraploids. Results underline that genetic factors have a predominant effect on the salt response, although a common stress response independent from genotype was also found.
URL: http://dx.doi.org/10.1016/j.jplph.2013.03.006
Subject Citrus Citrus reshni Citrus deliciosa Leaf proteome Polyploidy Salt stress

Icona documento 1) Download Document PDF

Icona documento Open access Icona documento Restricted Icona documento Private


Per ulteriori informazioni, contattare: Librarian http://puma.isti.cnr.it

Valid HTML 4.0 Transitional