Istituto di Biofisica     
Frassanito A. M., Barsanti L., Passarelli V., Evangelista V., Gualtieri P. A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system. In: Journal of Photochemistry and Photobiology B-Biology, vol. 125 pp. 188 - 193. Elsevier, 2013.
Here we report the identification and expression of a second rhodopsin-like protein in the alga Cyanophora paradoxa (Glaucophyta), named Cyanophopsin_2. This new protein was identified due to a serendipity event, since the RACE reaction performed to complete the sequence of Cyanophopsin_1, (the first rhodopsin- like protein of C. paradoxa identified in 2009 by our group), amplified a 619 bp sequence corresponding to a portion of a new gene of the same protein family. The full sequence consists of 1175 bp consisting of 849 bp coding DNA sequence and 4 introns of 326 bp. The protein is characterized by an N-terminal region of 47 amino acids, followed by a region with 7 a-helices of 213 amino acids and a C-terminal region of 22 amino acids. This protein showed high identity with Cyanophopsin_1 and other rhodopsin- like proteins of Archea, Bacteria, Fungi and Algae. Cyanophosin_2 (CpR2) was expressed in a cell-free expression system, and characterized by means of absorption spectroscopy.
URL: http://dx.doi.org/10.1016/j.jphotobiol.2013.06.010
Subject Rhodopsin-like protein Cyanophora paradoxa CpR2 Cell-free expression Absorption spectrum

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