PUMA
Istituto di Biofisica     
Di Garbo A., Alloisio S., Nobile M. P2X7 receptor-mediated calcium dynamics in HEK293 cells: experimental characterization and modelling approach. In: Physical Biology, vol. 9 pp. 26001 - 260013. IOP Publishing, 2012.
 
 
Abstract
(English)
The P2X7 receptor (P2X7R) induces ionotropic Ca2+ signalling in different cell types. It plays an important role in the immune response and in the nervous system. Here, the mechanisms underlying intracellular Ca2+ variations evoked by 3-O-(4-benzoyl)benzoyl-ATP (BzATP), a potent agonist of the P2X7R, in transfected HEK293 cells, are investigated both experimentally and theoretically. We propose a minimal model of P2X7R that is capable of reproducing, qualitatively and quantitatively, the experimental data. This approach was also adopted for the P2X7R variant, which lacks the entire C-terminus tail (trP2X7R). Then we introduce a biophysical model describing the Ca2+ dynamics in HEK293. Our model gives an account of the ionotropic Ca2+ influx evoked by BzATP on the basis of the kinetics model of P2X7R. To explain the complex Ca2+ responses evoked by BzATP, the model predicted that an impairment in Ca2+ extrusion flux through the plasma membrane is a key factor for Ca2+ homeostasis in HEK293 cells.
DOI: 10.1088/1478-3975/9/2/026001
Subject P2X7


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