PUMA
Istituto di Biofisica     
Frassanito A. M., Barsanti L., Evangelista V., Passarelli V., Gualtieri P. A rhodopsin-like protein in Cyanophora paradoxa: gene sequence and protein immunolocalization. In: Cellular and Molecular Life Sciences, vol. 67 pp. 965 - 971. Birkhašuser Verlag, 2010.
 
 
Abstract
(English)
Here, we report the DNA sequence of the rhodopsin gene in the alga Cyanophora paradoxa (Glaucophyta). The primers were designed according to the conserved regions of prokaryotic and eukaryotic rhodopsin- like proteins deposited in the GenBank. The sequence consists of 1,272 bp comprised of 5 introns. The correspondent protein, named Cyanophopsin, showed high identity to rhodopsin-like proteins of Archea, Bacteria, Fungi, and Algae. At the N-terminal, the protein is characterized by a region with no transmembrane a-helices (80 aa), followed by a region with 7a-helices (219 aa) and a shorter 35-aa C-terminal region. The DNA sequence of the N-terminal region was expressed in E. coli and the recombinant purified peptide was used as antigen in hens to obtain polyclonal antibodies. Indirect immunofluorescence in C. paradoxa cells showed a marked labeling of the muroplast (aka cyanelle) membrane.
DOI: DOI 10.1007/s00018-009-0225-x
Subject Rhodopsin-like protein  Cyanophora paradoxa  Muroplast  Cyanelle  Antibody labeling


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