Istituto di Biofisica     
Donadio E., Piccolomini F., Dimuccio V., Felicioli A., Balestreri E., Cianti R., Armini A., Bini L., Felicioli R., Donadio C. Serum albumin fragmentation in end-stage renal disease patients - a pilot study. In: Clinical Chemistry and Laboratory Medicine, vol. 47 (11) pp. 1373 - 1379. de Gruyter, 2009.
Background: The goal of this study was to detect modification in the expression of plasma proteins and/or post-translational modifications of their structure in patients with end stage renal disease. Methods: Serum samples from 19 adult patients treated by maintenance hemodialysis (MHD) were analyzed in comparison to sera from six healthy controls using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2DE). Spots of interest were identified by mass spectrometry analysis. In addition, the 2DE maps were incubated with a human anti-albumin polyclonal antibody. Results: SDS-PAGE gels, 2DE maps and matrix-assisted laser desorption/ionization time of flight analysis indicated over-expression of low-molecular weight proteins (LMWP) in sera from patients. Unexpectedly, another 15 spots with estimated Mr of 12.5-29 kDa from the 2DE maps of six patients were identified as fragments of albumin. 2D immunoblotting of sera from 12 other patients detected numerous albumin fragments. Conclusions: These results indicate that in addition to increased expression of LMWP, a relevant amount of albumin fragments are detectable in the serum of patients undergoing MHD. Uremia appears to facilitate the fragmentation of albumin and/or the retention of albumin fragments in blood.
URL: http://www.reference-global.com/toc/cclm/47/11
DOI: 10.1515/CCLM.2009.303
Subject albumin fragments
low-molecular weight proteins
matrix-assisted laser desorption/ionization time of flight mass spectrometry
plasma proteomics
uremic syndrome

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