PUMA
Istituto di Biofisica     
Evangelista V., Frassanito A. M., Passarelli V., Barsanti L., Gualtieri P. Microspectroscopy of the photosynthetic compartment of algae and plants. In: Photochemistry and Photobiology, vol. 82 (4) pp. 1039 - 1046. American Society for Photobiology, 2006.
 
 
Abstract
(English)
We performed microspectroscopic evaluation of the pigment composition of the photosynthetic compartments of algae belonging to different taxonomic divisions and higher plants. The feasibility of microspectroscopy for discriminating among species and/or phylogenetic groups was tested on laboratory cultures. Gaussian bands decompositions and a fitting algorithm, together with fourth-derivative transformation of absorbance spectra, provided a reliable discrimination among chlorophylls a, b and c, phycobiliproteins and carotenoids. Comparative analysis of absorption spectra highlighted the evolutionary grouping of the algae into three main lineages in accordance with the most recent endosymbiotic theories.
DOI: 10.1562/2006-01-11-RA-772
Subject Microspectroscopy
Algae
Pigment
Endosymbiosis
Gaussian decomposition
Fourth-derivative transformation


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