PUMA
Istituto di Biofisica     
Du Manoir S., Kallioniemi O., Lichter P., Piper J., Benedetti P. A., Carothers A., Fantes J., García-Sagredo J., Gerdes T., Giollant M., Hemery B., Isola J., Maahr J., Morrison H., Perry P., Stark M., Sudar D., Van Vliet L., Verwoerd N., Vrolijk J. Hardware and software requirements for quantitative analysis of comparative genomic hybridization. In: Cytometry Part A, vol. 19 (1) pp. 4 - 9. Wiley, 2005.
 
 
Abstract
(English)
Recommendations are made for hardware and software capabilities that will permit a level of performance of comparative genomic hybridization (CGH) analysis on metaphase chromosomes that is comparable to the best current practice. Guidelines for interpreting the results of CGH analysis in terms of chromosomal gains or losses are also presented
DOI: 10.1002/cyto.990190103
Subject Background correction
CCD camera
Filters
Fluorochromes
Image quality assessment
Intensity normalization
Pixel shift correction
Relative copy number karyotype


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