Istituto di Biofisica     
Loreti V., Toncelli D., Morelli E., Scarano G., Bettmer J. Biosynthesis of Cd-bound phytochelatins by Phaeodactylum tricornutum. In: Analytical and Bioanalytical Chemistry, vol. 383 pp. 398 - 403. Springer-Verlag, 2005.
Cd-bound phytochelatins (Cd-PCs) have been synthesised by incubation of Phaeodactylum tricornutum cell cultures with Cd and purified by size-exclusion chromatography-UV-Vis. These complexes, which were identified in previous work, have now been used as model substances to develop and optimise ion-pair chromatography (IPC) coupled to inductively coupled plasma-mass spectrometry (ICP-MS) for analysis of Cd-PCs. Subsequent analysis of samples taken from Silene vulgaris plants cultivated under heavy metal stress conditions revealed Cd signals but no Cd-PC signals. By use of isotopically enriched 116Cd-PCs the sample preparation steps were verified to determine the stability of the analytes. We observed species transformation between Cd-PCs and other unidentified Cd complexes. Consequently, the kinetic and thermodynamic lability of Cd-PCs are decisive factors in their detection.
DOI: 10.1007/s00216-005-3385-z
Subject ICP-MS SEC
Ion-pair chromatography
Phaeodactylum tricornutum
Silene vulgaris

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