PUMA
Istituto di Biofisica     
Solaini G., Baracca A., Gabellieri E., Lenaz G. Modification of the mitochondrial F1-ATPase e subunit, enhancement of the ATPase activity of the IF1-F1 complex and IF1-binding dependence of the conformation of the e subunit. In: Biochemical Journal, vol. 327 pp. 443 - 448. Biochemical Society, 1997.
 
 
Abstract
(English)
Treatment of bovine heart submitochondrial particles with a low concentration of 2-hydroxy-5-nitrobenzyl bromide (HNB), a selective reagent for the Trp residue of the e subunit [Baracca, Barogi, Lenaz and Solaini (1993) Int. J. Biochem. 25, 1269-1275], enhances the ATP hydrolytic activity of the particles exclusively when the natural inhibitor protein IF1 is present. Similarly, isolated F1 [the catalytic sector of the mitochondrial H+-ATPase complex (ATP synthase)] treated with the reagent has the ATPase activity enhanced exclusively if IF1 is bound to it. These experiments suggest that the modification of the e subunit decreases the inhibitory activity of IF1, eliciting the search for a relationship between the e subunit and the inhibitory protein. Certainly, a reverse relationship exists because HNB binds covalently to the isolated F1 exclusively when the inhibitory protein is present. This finding is consistent with the existence of the e subunit in different conformational states depending on whether IF1 is bound to F1 or not. Support for this assertion is obtained by measurements of the intrinsic phosphorescence decay rate of F1, a probe of the Trp e subunit conformation in situ [Solaini, Baracca, Parenti-Castelli and Strambini (1993) Eur. J. Biochem. 214, 729-734]. A significant difference in phosphorescence decay rate is detected when IF1 is added to preparations of F1 previously devoid of the inhibitory protein. These studies indicate that IF1 and the e subunit of the mitochondrial F1-ATPase complex are related, suggesting a possible role of the e subunit in the mechanism of regulation of the mitochondrial ATP synthase.
URL: http://www.biochemj.org/bj/327/bj3270443.htm
Subject Mitochondrial F1-ATPase
Trp phosphorescence
H+-ATPase complex inhibitor


Icona documento 1) Download Document PDF


Icona documento Open access Icona documento Restricted Icona documento Private

 


Per ulteriori informazioni, contattare: Librarian http://puma.isti.cnr.it

Valid HTML 4.0 Transitional