PUMA
Istituto di Biofisica     
Seritti A., Nannicini L., Del Vecchio R. Use of Synchronous Scan Excitation Spectra to Detect Fluorescent Metal Organic Ligands Produced by the Marine Diatom Phaeodactylum Tricornutum. In: Environmental Technology, vol. 17 (1) pp. 25 - 33. Taylor, 1996.
 
 
Abstract
(English)
The use of synchronous scan excitation spectra (SSES) for investigating the fluorescence characteristics of the Sep-Pak extractable phytoplanktonic organic matter (SPE-POM) produced by the marine diatom Phaeodactylum tricornutum under (SPE-POM*) and without (SPE-POM) metal stress is proposed. A Δλ of 70 nm was found suitable to detect the fluorophores occurring in such samples. A proteinaceous, lumichrome and lumiflavin contributions together with that of a degradation and/or condensation product were observed. The fluorescence quenching technique applied to SSES enabled detection of the contribution of each group of fluorophores to the copper complexation. The log K of complexes were in the range 5-6. A comparison with the Sep-Pak extractable marine organic matter (SPE-MOM) from seawater shows a relationship between the proteinaceous material produced by Phaeodactylum tricornutum and that occurring in seawater.
DOI: 10.1080/09593331708616357
Subject Metal complexation
DOM
Phytoplankton
Fluorescence


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