Istituto di Biofisica     
Seritti A., Nannicini L., Del Vecchio R. Use of Synchronous Scan Excitation Spectra to Detect Fluorescent Metal Organic Ligands Produced by the Marine Diatom Phaeodactylum Tricornutum. In: Environmental Technology, vol. 17 (1) pp. 25 - 33. Taylor, 1996.
The use of synchronous scan excitation spectra (SSES) for investigating the fluorescence characteristics of the Sep-Pak extractable phytoplanktonic organic matter (SPE-POM) produced by the marine diatom Phaeodactylum tricornutum under (SPE-POM*) and without (SPE-POM) metal stress is proposed. A Δλ of 70 nm was found suitable to detect the fluorophores occurring in such samples. A proteinaceous, lumichrome and lumiflavin contributions together with that of a degradation and/or condensation product were observed. The fluorescence quenching technique applied to SSES enabled detection of the contribution of each group of fluorophores to the copper complexation. The log K of complexes were in the range 5-6. A comparison with the Sep-Pak extractable marine organic matter (SPE-MOM) from seawater shows a relationship between the proteinaceous material produced by Phaeodactylum tricornutum and that occurring in seawater.
DOI: 10.1080/09593331708616357
Subject Metal complexation

Icona documento 1) Download Document PDF

Icona documento Open access Icona documento Restricted Icona documento Private


Per ulteriori informazioni, contattare: Librarian http://puma.isti.cnr.it

Valid HTML 4.0 Transitional