Istituto di Biofisica     
Herrmann H., Häder D., Köfferlein M., Seidlitz H., Ghetti F. Effects of UV radiation on photosynthesis of phytoplankton exposed to solar simulator light. In: Journal of Photochemistry and Photobiology B-Biology, vol. 34 (1) pp. 21 - 28. Elsevier, 1996.
A sunlight simulator was used to investigate the effects of short term irradiation on photosynthetic oxygen production and chlorophyll fluorescence in the unicellular algae Dunaliella salina and Ochromonas danica. Oxygen evolution was measured with a Clark-type electrode and initial (F0) and maximal (Fm) fluorescence were determined by means of a pulse amplitude-modulated (PAM) fluorometer. Using different long-pass filters, a UV exclusion study was performed comparing the effects of irradiation with full sun simulator light, with sun simulator light without UV-B and short wavelength UV-A radiation and without any UV component. In D. salina, exposure to full sun simulator light caused a marked drop in photochemical efficiency indicated by a decline in the initial slope of the irradiance-response curve for photosynthetic oxygen production and by a decrease in the ratio Fv/Fm, whereas only a slight inhibition of photosynthetic activity was observed irradiating with PAR and UV-A radiation or with PAR alone. In the case of O. danica, a strong inhibition was observed under all irradiation conditions. The results are consistent with preliminary data obtained with outdoor experiments.
DOI: 10.1016/1011-1344(95)07245-4
Subject Dunaliella salina
Oxygen evolution
Ochromonas danica
PAM fluorescence
Sunlight simulato
UV-B radiation
UV-A radiation

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