PUMA
Istituto di Biofisica     
Scarano G., Morelli E. Cathodic stripping voltammetric determination of Metallothioneins. In: Electroanalysis, vol. 8 (4) pp. 396 - 398. VCH, 1996.
 
 
Abstract
(English)
A cathodic stripping voltammetric method for the determination of metallothioneins (MT)is proposed. The procedure is based on the controlled adsorption of the protein on the mercury electrode after addition of an excess of CuII to the solution. The effects of accumulation time, adsorption potential and copper concentration have been examined and the analytical procedure has been optimized. Using an accumulation time of 300 s, a MT concentration of 0.2nM can be detected.
DOI: 10.1002/elan.1140080418
Subject Metallothioneins
Cathodic stripping voltammetry
Metal-binding proteins


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