PUMA
Istituto di Biofisica     
Benedetti P. A., Evangelista V., Guidarini D., Vestri S. Electronic multi-confocal-points microscopy. In: SPIE - Three dimensional microscopy: image acquisition and processing II : proceedings reprint of the International society of optical engineering (San JosŤ, California, 9-10 Febrary 1995). Proceedings, pp. 56 - 62. Carol J. Cogswell, Tony Wilson (eds.). Society of optical engineering, 1995.
 
 
Abstract
(English)
A method for confocal optical microscopy is presented in which the role usually played by spatial modulators (pinhole or fiber) placed before the detector, is electronically emulated by an adaptive pixel-exclusion process performed at the level of an image detector. The technique is optimally suited to the construction of systems based on a multi-point illumination and detection approach, with the aid of conventional lamps and CCD image sensors. Due to its inherent simplicity and versatility, the proposed design can be efficiently employed in transmission, reflection and fluorescence microscopy, using trans- or epi- illumination contlgurations. The resulting advantages appear particplarly relevant in biomedical applications, not only with the purpose of setting up practical and affordable instruments, but also in order to avoid specimen damageor signal saturation effects, as well as to obtain confocal images of light-absorbing microscopical structures.
Subject Optical microscopy
3D microscopy
Image processing
Trans-illumination
Epi-illumination
Pinhole
CCD sensor


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