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Istituto di Biofisica     
Frediani C., Ascoli C., Lucia S., Verkerk P., Guidoni L., Fioretti A., Arimondo E. Optical trapping and manipulation of single cells and motile microorganisms by laser diode radiation. In: Proceedings of biomedical optoelectronic devices ans systems II (Lille, France, 6-8 september 1994). Proceedings, pp. 161 - 166. Hans-Jochen Foth ... [et al.] (ed.). (Progress in biomedical optics : EurOpto series : SPIE proceedings series, vol. 2328). SPIE, 1994.
 
 
Abstract
(English)
Electromagnetic fields, emitte d by laser sources , have been utilized in recent years for controlling the position and velocity of atoms, ions and microscopic neutral particles. In 1987 Ashkin67 has shown for the first time that cells too can be trapped byusing a focused beam of laser radiation. The trappin is due to the to the interation between the electric dipole induced by the laser electric field in the cell and the electric field itself. In order to maximize the trapping effect and to avoid damage to the cells caused by excessive heating, the laser wavelength must be far from the absorption bands for both the cells and the an so1ution where cells are kept, usually water. Our preliminary experiments, utilizing a 100 mW 1aser diode; at 850 nm with suitable focusing, show that alsofree swimming (up to 100 m/s) protozoa(Dunaliella salina) can be easily trapped, without apparent damage. The experimental seteup and the experiments on motile microorganisms arepresented. Possible biomedical applications are discussed.
Subject Laser optical trapping
Motile microorganisms
Dunaliella salina
H. Salinarum


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