PUMA
Istituto di Biofisica     
Gualtieri P. Euglena gracilis: is the photoreception enigma solved?. In: Journal of Photochemistry and Photobiology B-Biology, vol. 19 (1) pp. 3 - 14. Elsevier, 1993.
 
 
Abstract
(English)
Since the turn of the century the photosynthetic and photosensitive flagellate Euglena gracilis has provided an elective point for photobiological studies. Indeed, it plays a unique evolutionary role in photoreception, as its paraflagellar swelling can be considered as the first ciliary photoreceptor consisting of a natural three-dimensional crystal of a membrane protein. Previous fluorescence and action spectra studies on the nature of the pigment content of this organelle suggested a flavin nature of its chromophores. Recently, theoretical considerations, absorption microspectroscopic measurements, inhibition experiments on the biosynthesis of carotenoids, the photobehaviour in the presence of hydroxylamine hydrochloride, and the identification of all-trans-retinal by thin layer chromatography, high performance liquid chromatography (HPLC), absorption spectroscopy and gas chromatographyŚmass spectrometry (GC---MS) have indicated that rhodopsin is the photoreceptive molecule of this microorganism. All of these studies are compared and critically discussed on the basis of newly collected experimental data.
DOI: 10.1016/1011-1344(93)80086-O
Subject Euglena gracilis
Photoreception
Rhodopsin
Flavin
Signal transduction


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